Upper panel: IFA images of mature cycle schizonts, showing normal parasite development and RAP-induced loss of SUB1HA3 expression (representative of six independent experiments). Loss of SERA6 expression could not be similarly confirmed by IFA due to C-terminal tagging of SERA6 b eing unsuccessful and the lack of suitable SERA6-specific antibodies. Scale bar, 5 μm. DAPI, 4,6-diamidino-2-phenylindole. Both SUB1-null (ΔSUB1) and ΔSERA6 parasites formed morphologically normal schizonts at the end of cycle 0, showing that neither gene is required for intracellular development
Lower panel: Microneme discharge in ΔSUB1 parasites. IFA of C2-arrested parasites compared to 30 min after washing away C2. Translocation of AMA1 to the intracellular merozoite surface is evident in the washed parasites. Scale bar, 10 μ m. Microneme discharge occurred in the trapped ΔSUB1 merozoites. Similar to SUB1 discharge, AMA1 discharge is blocked by PKG inhibitors, so this confirmed reversal of the C2-mediated PKG inhibition in these experiments.
Thomas JA, Tan MYS, Bisson C, Borg A, Umrekar TR, Hackett F, Hale VL, Vizcay-Barrena G, Fleck RA, Snijders AP, Saibil HR, Blackman MJ. A protease cascade regulates release of the human malaria parasite Plasmodium falciparum from host red blood cells. Nat Microbiol. 2018 Feb 19.. [Epub ahead of print] PMID: 29459732.
Other associated proteins
|PF3D7_0507500||subtilisin-like protease 1|
|PF3D7_0930300||merozoite surface protein 1|