Confocal microscopy demonstrates the localization of BAEBL in micronemes. (A) Dd2yNm schizonts were double labeled with anti-BAEBL region 2 and anti-EBA-175. Schizonts immunolabeled with anti-BAEBL region 2 were stained with Alexa 488 secondary antibody (green). Schizonts labeled with anti-EBA-175 were stained with Alexa594 secondary antibody (red). (B)Dd2yNm schizonts were double labeled with anti-BAEBL region 6 and anti-EBA-175. Schizonts immunolabeled with anti-BAEBL region 6 were stained with Alexa 488 secondary antibody (green). Schizonts labeled with anti-EBA-175 were stained with Alexa 594 secondary antibody (red). (C) Dd2yNm schizonts were double labeled with anti-BAEBL region 2 and anti-RAP-1 monoclonal antibody. Schizonts immunolabeled anti-BAEBL region 2 were stained with Alexa 488 secondary antibody (green). Schizonts labeled with anti-RAP-1 were stained with tetramethyl rhodamine isothiocyanate (TRITC) secondary antibody (red). (D) Dd2yNmschizonts were double labeled with anti-BAEBL region 6 and anti-RAP-1 monoclonal antibody. Schizonts immunolabeled with anti-BAEBL region 6 were stained with Alexa 488 secondary antibody (green). Schizonts labeled with anti-RAP-1 were stained with TRITC secondary antibody (red). Antibodies to regions 2 and 6 localize to the same organelle as EBA-175 (A and B), which has been shown to localize to the micronemes. Furthermore, immunolocalization of RAP-1, a protein found in rhoptries, other apical organelles of merozoites, shows that BAEBL is adjacent to but not overlapping RAP-1 (C and D). This distribution is consistent with the localization of BAEBL within micronemes, a distribution identical to that of EBA-175.

Mayer DC, Kaneko O, Hudson-Taylor DE, Reid ME, Miller LH. Characterization of a Plasmodium falciparum erythrocyte-binding protein paralogous to EBA-175. Proc Natl Acad Sci U S A. 2001 98(9):5222-7.