3D-SIM observations of episomally expressed PTP5 and PTP5ΔPHR. (a) Subcellular localization of PTP5FL-HA (green) and (b) PTP5ΔPHR-HA (green) observed with SIM. EXP2 (red) and PfPV1 (cyan) were used as a counter-staining.. (d) Schematic presentation of PTP5FL-HA (upper panel) and PTP5ΔPHR-HA (lower panel). PEXEL motif, PV1 high-affinity region (PHR), and HA tag of episomally expressed PTP5 are represented in orange, gray, and green colors respectively. The present study identified PHR as essential for PTP5 export, suggesting that PV1 plays an important role in protein translocation mediated by the PTEX complex. The PTP5FL-HA that interacted with PV1 successfully translocated to the cytosol of infected erythrocyte. PTP5ΔPHR-HA mutant weakly interacts PV1 and the export of this protein was arrested at PV.

Morita M, Nagaoka H, Ntege EH, Kanoi BN, Ito D, Nakata T, Lee JW, Tokunaga K, Iimura T, Torii M, Tsuboi T, Takashima E. PV1, a novel Plasmodium falciparum merozoite dense granule protein, interacts with exported protein in infected erythrocytes. Sci Rep. 2018 8(1):3696.