Characterization of 3D7-PfCDPK5DDKnL parasites and PfCDPK5 localization. Schizonts from [+] Shld-1 3D7-PfCDPK5DDKnL parasites were fixed, probed with anti-HA and anti-PfAMA1 antibodies, and visualized by SR-SIM. Bars, 2 mm. PfCDPK5 retained colocalization withPfAMA1 but also exhibited additional diffuse apical staining (third row). In parasites that were beyond the “egress trigger,” PfAMA1 was translocated to the merozoite surface, and PfCDPK5 relocalized to a peripheral cytoplasmic distribution with increased signal near the plasma membrane (E64-treated row). Localization was similar in free merozoites isolated from parallel cultures grown without E64 (Free merozoite row).

Absalon S, Blomqvist K, Rudlaff RM, DeLano TJ, Pollastri MP, Dvorin JD. Calcium-Dependent Protein Kinase 5 Is Required for Release of Egress-Specific Organelles in Plasmodium falciparum. MBio. 2018 9(1). pii: e00130-18