Expression and localization of Puf3. Co-localization of PfPuf3 (green) and the nucleolar marker PfNop1 (red) was observed in ring (R), early trophozoite (ET), late trophozoite (LT) and schizont (S) stages. Nuclei were stained with Hoechst 33342. Using the PfPuf3::GFP line with PfNop1-tdTomato expressed from a nuclear plasmid, we found excellent overlap of these two tagged proteins in ring, trophozoite and schizont stages that PfPuf3 is a nucleolar protein.

Liang X, Hart KJ, Dong G, Siddiqui FA, Sebastian A, Li X, Albert I, Miao J, Lindner SE, Cui L. Puf3 participates in ribosomal biogenesis in malaria parasites. J Cell Sci. 2018 Feb 27. [Epub ahead of print]