PTRAMP is a merozoite surface protein that originates in the micronemes. Immunofluorescence assays were performed on fixed smears of mixed stage parasites using affinity purified PTRAMP antisera in conjunction with sera detecting the microneme protein AMA-1 or the rhoptry protein RAP1. Black and white images show the immunofluorescence pattern detected with the designated antisera or of the parasite nuclei visualised with DAPI. (Panel 1a) PTRAMP (green fluorescence) is detected in the apical organelles (arrowed) of P. falciparum IT0/A4 early intraerythrocytic schizonts (s) and at the merozoite surface in mature segmenting schizonts (ss). Earlier stage parasites, including rings (r) do not express PTRAMP. (Panel 1b) PTRAMP is expressed at the surface of P. berghei merozoites. (Panel 2a) PTRAMP (red fluorescence) co-localises with AMA-1 (green fluorescence) in the developing micronemes (arrowed) of 3D7 P. falciparum intraerythrocytic schizonts that are still undergoing DNA replication. (Panel 2b) PTRAMP is detected at the surface of released merozoites (m) following schizont rupture whilst AMA-1 localises within the micronemes. (Panel 3a) PTRAMP (red fluorescence) shows a distinct localisation pattern from RAP1 (green fluorescence) in 3D7 P. falciparum intraerythrocytic schizonts. (Panel 3b) PTRAMP is detected at the surface of released merozoites (m) following schizont rupture whilst RAP1 localises within the rhoptries.
Thompson J, Cooke RE, Moore S, Anderson LF, Janse CJ, Waters AP. PTRAMP; a conserved Plasmodium thrombospondin-related apical merozoite protein. Mol Biochem Parasitol. 2004 Apr;134(2):225-32.
Other associated proteins
|PF3D7_1218000||thrombospondin-related apical membrane protein|