PF3D7_1203000 origin recognition complex subunit 1

Orc2 goes to the nucleus through the nuclear pore. (A) Orc2 showed a diffused pattern upon Ivermectin treatment. Ring (18±4 hpi) parasites were incubated with 10μM of Ivermectin along with DMSO as a vehicle control for 20 hours. SSB (a non-nuclear protein like apicoplast targeted SSB) was used as a nonnuclear negative control. (B) Orc1 as a positive control showed similar diffusion upon Ivermectin treatment which confirms that the diffusion of Orc2 is due to an inhibition of nuclear import (C) 3D reconstruction of Z stacked images showed the spatial localization of Orc2 with respect to the nucleus. Ivermectin treatment resulted in the loss of punctate foci as seen in the vehicle control lane. The white arrow shows the boundary of the nucleus seen due to the inability of Orc2 to enter the nucleus. (n>7) ~300 cells were analysed in this study. DAPI was used to stain the nucleus.

Sharma R, Sharma B, Gupta A, Dhar SK. Identification of a novel trafficking pathway exporting a replication protein to nucleus via classical secretory pathway in Plasmodium falciparum. Biochim Biophys Acta. 2018 Mar 7. [Epub ahead of print]

Other associated proteins

PFID Formal Annotation
PF3D7_0705300 mediator of RNA polymerase ii transcription subunit 10, putative