PF3D7_1133400 apical membrane antigen 1

The effect of the absence of GP1 and GP2 on some organelle markers observed by IFA. Immunofluorescence assay on wildtype parasites (GP1-3HA-glmS 0mM GlcN) and on parasites lacking both proteins (KO GP2 in GP1-3HA-glmS 2.5mM GlcN). Markers for different parasite organelles were tested in combination with an anti-HA. Scale bar=5 µm. Investigation by IFA whether the localisation of different organellar markers was affected in the KD/KO line. Only some minor effects were noticeable for the microneme markers AMA1 and EBA175, the inner membrane complex protein GAP45 and the merozoite surface protein MSP1 Globally, the lack of any obvious specific defect might suggest that general parasite fitness is affected.

Hallée S, Theriault C, Gagnon D, Kehrer J, Frischknecht F, Mair GR, Richard D.Identification of a Golgi apparatus protein complex important for the asexual erythrocytic cycle of the malaria parasite Plasmodium falciparum. Cell Microbiol.2018 Mar 26:e12843.

Other associated proteins

PFID Formal Annotation
PF3D7_0207600 serine repeat antigen 5
PF3D7_0501600 rhoptry-associated protein 2
PF3D7_0731500 erythrocyte binding antigen-175
PF3D7_0930300 merozoite surface protein 1
PF3D7_1116000 rhoptry neck protein 4
PF3D7_1320000 rhoptry protein 2, putative golgi protein 1
PF3D7_1353600 ER lumen protein retaining receptor