Colocalization analysis of the exosomal proteins in the parasite by indirect immunofluorescent assay. The red fluorescence (Alexa Fluor 594) indicates PfDis3 and the green fluorescence (Alexa Fluor 488) indicates PfRrp4, PfRrp41, PfRrp42 and PfRrp6. Scale bar, 5 μm. The colocalization of these components in the periphery of the nucleus indicated that they are essential elements in the complex. However, unlike that of Dis3 in human and yeast, which are mainly nuclear, PfDis3 is only cytoplasmic, indicating that PfDis3 is involved in the composition of the cytoplasmic complex. Further, Rrp6 has previously been considered as the main component of nuclear exosome; PfRrp6, however, appeared in the cytoplasm.
Jiang N, Yu S, Yang N, Feng Y, Sang X, Wang Y, Wahlgren M, Chen Q. Characterization of the Catalytic Subunits of the RNA Exosome-like Complex in Plasmodium falciparum. J Eukaryot Microbiol. 2018 Apr 17 [Epub ahead of print]
Other associated proteins
|PF3D7_1340100||exosome complex component RRP42, putative|
|PF3D7_1427800||exosome complex exonuclease RRP41, putative|
|PF3D7_1449700||exosome complex exonuclease RRP6|