Immunofluorescence images showing trafficking of the 3D7-miniSOG-FLAG CLAG3 isoform in miniSOG2. This protein, expressed under the msp2 promoter, colocalizes with RhopH3 in schizonts and is exported normally in trophozoites after invasion of new erythrocytes. Bar, 5 mm. the 3D7 allele carried the same tandem miniSOG-FLAG epitope tag at its C terminus as in TFLC3 but with expression under a heterologous msp2 promoter. We selected this promoter

because msp2 exhibits a stage-specific transcription profile similar to that of clag genes in P. falciparum. This choice yielded CLAG3 expression and faithful trafficking to the rhoptry within schizonts and export to the host compartment in trophozoites.

Gupta A, Balabaskaran-Nina P, Nguitragool W, Saggu GS, Schureck MA, Desai SA. CLAG3 Self-Associates in Malaria Parasites and Quantitatively Determines Nutrient Uptake Channels at the Host Membrane. MBio. 2018 9(3).