PF3D7_0404700 dipeptidyl aminopeptidase 3

DPAP3 is secreted at the time of egress. IEM section obtained from DPAP3-GFP parasites. Close-up mages of individual intracellular merozoites on the left show immunogold staining of DPAP3-GFP in close proximity to the rhoptries (green arrows) and at the apical end of merozoites (blue arrows). Images on the right show representative sections of schizonts with an intact (black arrows) or rupture PVM. Staining of extracellular DPAP3-GFP (white arrows) was only observed in schizonts lacking a PVM. Rhoptries (r), nuclei (n), and the RBCM (red arrows) are indicated. Rabbit anti-GFP and colloidal gold-conjugated anti-rabbit antibodies were used. Bar graph = 200 nm. Almost no DPAP3 staining was detected in the PV of schizonts with an intact PVM.

Lehmann C, Tan MSY, de Vries LE, Russo I, Sanchez MI, Goldberg DE, Deu E. Plasmodium falciparum dipeptidyl aminopeptidase 3 activity is important for efficient erythrocyte invasion by the malaria parasite. PLoS Pathog. 2018 [Epub ahead of print]

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