Distribution of the protein on merozoite surface. A, The distribution of the PfTrx-like-mero protein on merozoite in the schizont and before erythrocyte invasion was localized by indirect immunofluorescence. Nuclei are stained with Hoechst (blue color). The PfTrx-like-mero protein (PF3D7_1104400) is illustrated by green fluorescence-labelled antibodies, which was mostly seen on the periphery of the distribution of the PfTrx-like-mero protein is co-localized with MSP-1 during the asexual stage. After merozoite formation, the two proteins are distributed on the surface of both free and schizontic merozoites. At the trophozoite stage, the two proteins are distributed on the parasitophorous vacuole (PV) membrane and also inside the PV. Scale bar, 5 μm. C and D, Localization of the PfTrx-like-mero protein by immune-electronic microscopy. Parasites at late schizont (C) and free merozoites (D) were labeled using a protein specific antibody, and detected with a secondary antibody conjugated with gold particles. Gold particles were highlighted with red dashed frames. The protein is mostly localized on merozoite surface. Scale bar, 200

nm.

Wang W, Huang P, Jiang N, Lu H, Zhang D, Wang D, Zhang K, Wahlgren M, Chen Q. A thioredoxin homologous protein of Plasmodium falciparum participates in erythrocyte invasion. Infect Immun. 2018 May 29. [Epub ahead of print]