Subcellular localization of native Plasmodium falciparum surface-related antigen (PfSRA) in asexual stages. Methanol-fixed 3D7 ring, trophozoite, schizont, and released merozoites were stained with α-PfSRA antibodies. D, PfSRA does not colocalize with PfAMA-1 on the merozoite surface. Colabeling of rabbit α-PfSRA-1 (red) with mouse α-PfAMA-1 (1:100; green) antibodies in intact schizonts show that PfSRA is localized on the merozoite surface. Secondary antibodies used are Alexa 488–conjugated goat α-mouse immunoglobulin G (IgG), Alexa 568-conjugated goat α-rabbit IgG (1:200; Life Technologies). Exposure times were identical for all images of the same channel. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). E, PfSRA colocalizes with PfMSP119 at the parasitophorous vacuolar membrane in late trophozoites and on the merozoite surface. Colabeling of rabbit α-PfSRA-1 (1:100; red) with mouse α-PfMSP119 (1:100; green) antibodies in late trophozoites and intact schizonts show that PfSRA is localized at the PV and on the merozoite surface. Colocalization coefficient (R) is displayed at the far right. Secondary antibodies used are Alexa 488–conjugated goat α-mouse IgG and Alexa 568–conjugated goat α-rabbit IgG (1:200; Life Technologies). Exposure times are identical for all images of the same channel. Nuclei were stained with DAPI (blue). Abbreviations: BP, brightfield; DAPI, 4′,6-diamidino-2-phenylindole; PfSRA, Plasmodium falciparum surface-related antigen.
Amlabu E, Mensah-Brown H, Nyarko PB, Akuh OA, Opoku G, Ilani P, Oyagbenro R, Asiedu K, Aniweh Y, Awandare GA. Functional Characterization of Plasmodium falciparum Surface Related Antigen (PfSRA) as a Potential Blood-Stage Vaccine Target. J Infect Dis. 2018 Apr 18. [Epub ahead of print] PMID: 29912472
Other associated proteins
|PF3D7_0930300||merozoite surface protein 1|
|PF3D7_1431400||surface-related antigen SRA|