Localization of wild type and mutant PfDJ1 in parasites. Wild type P. falciparum 3D7 parasites (anti-DJ1) were assessed for expression of native DJ1 by IFA using anti-PfDJ1 antibodies. Live recombinant parasites were evaluated for expression of GFP/DJ1WT (DJ1WT) or mutants (C106A, S151A) using a fluorescence microscope. The images show the presence of native DJ1 or GFP/DJ1 specific signal (DJ1), staining of the nucleus (Nuc), parasite and the infected erythrocyte boundaries (Bright), and all three images overlapped (Merged). Intense diffuse signal in all the parasites indicates expression of DJ1 proteins.
Singhal N; Atul, Mastan BS, Kumar KA, Sijwali PS. Genetic ablation ofplasmoDJ1, a multi-activity enzyme, attenuates parasite virulence and reduces oocyst production. Biochem J. 2014 Jul 15;461(2):189-203.