Colocalization of PfMYST and H3K9me1 histone. Colocalization studies were performed by immunofluorescence assay in ring stage of parasite. Red color shows localization of PfMYST while green color depicts localization of H3K9me1 histone and AcH4 respectively. Blue color depict DAPI stained nucleus. PfMYST and H3K9me1 showed clear colocalization in the parasitophorus vacuole only in the ring stage. However, in other stages H3K9me1 exhibited dispersed signal in cytosol and nucleus and did not colocalize significantly with PfMYST (data not shown). For comparison, when we performed similar colocalization experiment of PfMYST with acetylated histone 4 (AcH4), we failed to observe PfMYST colocalization with AcH4 in the ring stage and both the proteins showed distinct distribution pattern where PfMYST was localized in the parasitophorous vacuole while AcH4 was localized exclusively in the nucleus. Together, these results show that PfMYST and H3K9me1 histone specifically colocalize with each other in ring stage of developmental cycle.

Sen U, Saxena H, Khurana J, Nayak A, Gupta A. Plasmodium falciparum RUVBL3 protein: a novel DNA modifying enzyme and an interacting partner of essential HAT protein MYST. Sci Rep. 2018 Jul 19;8(1):10917.