Stage-specific localization of PfRUVBL3 protein. Immunofluorescence assays were performed using anti-PfRUVBL3 antibody or pre-immune on fixed
smears of infected erythrocytes harboring various parasite stages of IDC. Green signal depicts localization of PfRUVBL3 protein while blue signal of DAPI stains nucleus. No signal was detected with pre-immune sera under similar experimental conditions. The bar as shown in inset is equivalent to 2 μM. PfRUVBL3 proteins localized in the parasitophorous vacuole of the parasite in the ring stage. However, in trophozoite stage, PfRUVBL3 signal majorly overlapped with DAPI while in schizont stage, it remained predominantly localized at the periphery of infected erythrocyte. Together, these results show that PfRUVBL3 protein expresses in all the stages right from ring to schizont however, its localization varies with stage progression of the parasite.
Sen U, Saxena H, Khurana J, Nayak A, Gupta A. Plasmodium falciparum RUVBL3 protein: a novel DNA modifying enzyme and an interacting partner of essential HAT protein MYST. Sci Rep. 2018 Jul 19;8(1):10917.