Left: Dissecting the PMV–PfSPC25 interaction. a, A schematic of the SPC25-GFP chimaera (1–179_GFP, 1–105_GFP and 46–179_GFP) and immunofluorescence analysis of all constructs showing that fluorescence localized to the ER. The transmembrane domains are shaded in grey. Nuclei were stained using DAPI. Scale bar, 5 μ m. N = 3 biologically independent samples used. All chimaeras had similar localization to the ER. Right: Characterization of the signal peptidase catalytic subunit PfSPC21. Immunofluorescence analysis of SPC21-HA parasites with anti-HA antibodies. SPC21-HA is colocalized at the ER with anti-SPC25 (top row) and anti-PMV (bottom row). Nuclei were localized with DAPI. Scale bars, 1 μ m. More than ten parasites were imaged for each condition. N = 3 biologically independent samples used.

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