EXP2 is essential for blood stage survival and protein export. a, Schematic of the TetR–DOZI–aptamer strategy for conditional translational repression of EXP2. 2A, Thosea asigna virus 2A skip peptide; HA, haemagglutinin tag; BSD, blasticidin-S deaminase; TetR–DOZI, tetracycline repressor–DOZI fusion d, e, Immunofluorescence assay showing export of the Plasmodium Export Element (PEXEL)-containing protein HRP2 (d) and the PEXEL negative exported protein (PNEP) SBP1 (e) in EXP2apt parasites (bearing a 3× Flag tag on HSP101) synchronized to a 3 h invasion window and allowed to develop for 24 h post-invasion with or without aTc. Merge images include aldolase (d) or HSP101-3× Flag (e) in green and DAPI in blue. Scale bars, 5 μm. f, g, Parasitophorous vacuolar morphological abnormalities following EXP2 knockdown (f) or HSP101 inactivation (g) visualized by transmission electron microscopy. The phenotype was visualized in three independent experiments by Giemsa stain and in a single electron microscopy experiment. Scale bars, 500 nm. Depletion of EXP2 resulted in a severe defect in the export of effector proteins beyond the PVM (Fig. 1d,e). This was accompanied by abnormalities in parasitophorous vacuole morphology that could be visualized by transmission electron microscopy to be tubular distensions of the PVM projecting into the erythrocyte cytosol, in contrast to the normal tight apposition of the PVM to the parasite plasma membrane
Garten M, Nasamu AS, Niles JC, Zimmerberg J, Goldberg DE, Beck JR. EXP2 is a nutrient-permeable channel in the vacuolar membrane of Plasmodium and is essential for protein export via PTEX. Nat Microbiol. 2018 .
Other associated proteins
|PF3D7_0831800||histidine-rich protein II|
|PF3D7_1116800||heat shock protein 101 chaperone protein ClpB2|