PF3D7_0721100-GFP parasites were stained with an antibody against the apicoplast marker ACP. Arrowheads indicate regions where PF3D7_0721100-GFP puncta appear adjacent to as opposed to colocalizing with ACP. Scale bars, 5 μm. ACP, acyl carrier protein; GFP, green fluorescent protein. PF3D7_0721100 localized to few large puncta not previously described for any apicoplast protein, which partly colocalized with the apicoplast marker ACP but also appeared adjacent to ACP staining (arrowheads).

Boucher MJ, Ghosh S, Zhang L, Lal A, Jang SW, Ju A, Zhang S, Wang X, Ralph SA, Zou J, Elias JE, Yeh E. Integrative proteomics and bioinformatic prediction enable a high-confidence apicoplast proteome in malaria parasites. PLoS Biol.

2018 16(9):e2005895.