The promiscuous biotin ligase BirA* biotinylates proteins in the P. falciparum apicoplast and ER. (B) Fixed-cell imaging of BioID±Ap and BioID±ER parasites stained with antibodies raised against the apicoplast marker ACP or the ER marker BiP, respectively. Scale bars, 5 μm. (D) Fixed-cell imaging of biotinylated proteins in biotin-labeled BioID±Ap and BioID±ER parasites. Scale bars, 5 μm. Immunofluorescence colocalization and live imaging of these parasites confirmed GFP±BirA* localization to either the apicoplast or the ER,
Boucher MJ, Ghosh S, Zhang L, Lal A, Jang SW, Ju A, Zhang S, Wang X, Ralph SA, Zou J, Elias JE, Yeh E. Integrative proteomics and bioinformatic prediction enable a high-confidence apicoplast proteome in malaria parasites. PLoS Biol. 2018 16(9):e2005895. PMID: 30212465.
Other associated proteins
|PF3D7_0208500||acyl carrier protein|
|PF3D7_1026900||biotin-protein ligase 1|