PfAtg5 expression is induced by short-term starvation and inhibited by 3-Methyladenine (3-MA). Immunofluorescence staining of PfAtg5 in parasites incubated in control, starvation, control+ 3-MA or starvation +3-MA media for 2 h. Representative image from at least 20 parasites is shown. Hoechst was used as DNA marker. Scale bar: 5 μm. we observed that PfAtg5

expression too increased upon starvation for brief periods

(2 h, Fig. 6). This result was reflected in the appearance of

PfAtg5 vesicles in late trophozoites/schizonts that were

subjected to starvation in presence or absence of autophagy

inhibition by 3-MA. Starvation caused an increase in abundance of PfAtg5 puncta while it diminished upon 3-MA treatment in normal growth conditions or during starvation.

Joy S, Thirunavukkarasu L, Agrawal P, Singh A, Sagar BKC, Manjithaya R, Surolia N. Basal and starvation-induced autophagy mediates parasite survival during intraerythrocytic stages of Plasmodium falciparum. Cell Death Discov. 2018 5:43. eCollection 2018.