Synchronized parasites at trophozoite stage were immunolabeled with anti-PfAtg5 antibodies (1:400) along with various organelle markers: Anti-KDEL antibody (1:200) as an endoplasmic reticulum marker, Anti-PfSSB antibody (1:200) as an apicoplast marker, MitoTracker Red CMXROS (100 nM) as a marker for mitochondria and Anti-PfAtg8 antibody as a marker for autophagosomes. Hoechst was used as DNA marker; Scale bar: 5 μm. PfAtg5 partially colocalized with ER, mitochondria, apicoplast and PfAtg8.

Joy S, Thirunavukkarasu L, Agrawal P, Singh A, Sagar BKC, Manjithaya R, Surolia N. Basal and starvation-induced autophagy mediates parasite survival during intraerythrocytic stages of Plasmodium falciparum. Cell Death Discov. 2018 5:43. eCollection 2018.