Knockdown of PTEX proteins EXP2 and PTEX150 reduces protein export into host erythrocytes. Export of Maurer’s cleft SBP1 protein after one cycle of GlcN treatment at 8 hpi rings by widefield microscopy in (A) 3D7, (B) EXP2-HAglmS and (C) PTEX150-HAglmS parasites. Representative immunofluorescence images showing reduced export of SBP1 structures after treatment of parasites with 0, 0.5 and 2 mMGlcN. Parasites were probed with rabbit anti-SBP1 serum (green), EXP2 mouse monoclonal IgG (red) and merged with DAPI DNA stain (blue). BF, brightfield. The brightness and contrast for SBP1 and EXP2 were equally adjusted for each image based on 3D7, 0 mMGlcN.

Charnaud SC, Kumarasingha R, Bullen HE, Crabb BS, Gilson PR. Knockdown of he translocon protein EXP2 in Plasmodium falciparum reduces growth and protein export. PLoS One. 2018 13(11):e0204785.