Gene editing and phenotyping of P. falciparum 7G8 strain to eliminate PfRh2a and PfRh2b expression.PfRh2b and PfRh2a were targeted in 7G8 using CRISPR/Cas9 technology and several clones were obtained from the resulting transfectant population. A) Expression of PfRh2b and PfRh2a genes for the parental lines 7G8 and GB4 and the edited 7G8 clones. B) Immunofluorescence analysis of the parental lines 7G8 and GB4 and two of the edited 7G8 clones using a specific anti-Rh2b antibody (green), nuclei are labelled with DAPI (blue).
Campino S, Marin-Menendez A, Kemp A, Cross N, Drought L, Otto TD, Benavente ED, Ravenhall M, Schwach F, Girling G, Manske M, Theron M, Gould K, Drury E, Clark TG, Kwiatkowski DP, Pance A, Rayner JC. A forward genetic screen reveals a primary role for Plasmodium falciparum Reticulocyte Binding Protein Homologue 2a and 2b in determining alternative erythrocyte invasion pathways. PLoS Pathog. 2018 14(11):e1007436.
Other associated proteins
|PF3D7_1335300||reticulocyte binding protein 2 homologue b, ALKBH5|