E5-HA-DD cultures with PfAP2-G stabilized at the ring stage (+ Shld) form stage I gametocytes before re-invasion. Gametocytes were detected as mononucleated parasites positive for the early gametocyte marker Pfg27. Similar results were obtained in four independent experiments. Scale bar, 5 μ m. Bottom” FACS-sorted PfAP2-G–eYFP-negative schizonts of the E5-eYFP line produce gametocytes within the first cycle after re-invasion.

Bancells C, Llorà-Batlle O, Poran A, Nötzel C, Rovira-Graells N, Elemento O, Kafsack BFC, Cortés A. Revisiting the initial steps of sexual development in the malaria parasite Plasmodium falciparum. Nat Microbiol. 2018