PF3D7_1017300 golgi re-assembly stacking protein 2

Right panel: Location of 2xFKBP-GFP-tagged PfVPS45 expressed from the endogenous locus (see also Figure S1). Arrowheads show signals at the food vacuole. The endogenous vps45 gene was tagged with the sequence encoding 2xFKBP-GFP using SLI (selection-linked integration). The corresponding cell line was successfully established and showed that VPS45 was present in foci and accumulations in the parasite cytoplasm in addition to a pool of VPS45 that was evenly distributed in the parasite cell. Bottom panel: Co-localization of PfVPS45-2xFKBP-GFP (VPS45-GFP) with mCherry-tagged P40PX (top, P40-mCh) and GRASP (bottom, GRASP-mCh). The signal at the food vacuole co-localized with the food vacuole marker P40PX, whereas other foci were found in close proximity to or overlapping with the Golgi marker GRASP.

Jonscher E, Flemming S, Schmitt M, Sabitzki R, Reichard N, Birnbaum J,

Bergmann B, Höhn K, Spielmann T. PfVPS45 Is Required for Host Cell Cytosol Uptake by Malaria Blood Stage Parasites. Cell Host Microbe. 2018 pii:S1931-3128(18)30592-4

Other associated proteins

PFID Formal Annotation
PF3D7_0216400 vacuolar protein sorting-associated protein 45, putative
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