PF3D7_0631900 stevor PIR protein

Left: Immunofluorescence analysis of the SFM+ parasite line cultured during 21 generations in the presence (SFM+, top panel) or absence (SFM-, bottom panel) of 40 nM pyrimethamine. Infected erythrocytes were stained with anti-c-myc mAb followed by anti-rat Alexa 488-conjugated IgG, and parasite nuclei were counterstained with Hoechst 33342. Pictures were taken under identical exposure conditions. Absence of c-myc expression in SFM- line indicates a loss of episomal expression of the epitope-tagged STEVOR protein. Bottom: Scanning electron micrograph of B3, SFM+ and SFM- parasite-infected erythrocytes. The right and middle panels show SFM- and SFM+ infected erythrocytes with normal knobs compared to erythrocyte infected with the KAHRP-deficient B3 parasite line (left panel) in which knobs are absent. The bars represent 2 μm.

Sanyal S, Egée S, Bouyer G, Perrot S, Safeukui I, Bischoff E, Buffet P, Deitsch KW, Mercereau-Puijalon O, David PH, Templeton TJ, Lavazec C. Plasmodium falciparum STEVOR proteins impact erythrocyte mechanical properties. Blood. 2012 119(2):e1-8.

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