PF3D7_0208500 acyl carrier protein

Stabilization of ACPL-GFP-mDHFR disrupts localization of endogenous apicoplast cargo. ACPL-GFP-mDHFR parasites were grown with 10 nM WR99210 and 200mM IPP for either 1 or 3 days. (A) Live imaging of Hoechst 33342-stained parasites. Bars, 5mm. (B) Fixed imaging of parasites stained with an antibody against the endogenous apicoplast marker ACP. The ACP antibody was raised against amino acids 57 to 137 of ACP (58) and therefore recognizes epitopes distinct from the first 55 amino acids that constitute the ACP leader sequence. Bars, 5mm.

Boucher MJ, Yeh E. Disruption of Apicoplast Biogenesis by Chemical Stabilization of an Imported Protein Evades the Delayed-Death Phenotype in Malaria Parasites. mSphere. 2019 4(1). pii: e00710-18.

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