Colocalisation in schizonts by IFA (following inhibitor treatment, left) of PfPDEβ-HA (red) with markers of specific subcellular compartments (green): plasmepsin V (PMV; ER marker), AMA1 (microneme marker), GAP45 (IMC marker), and MSP119 (plasma membrane marker). Nuclear material was visualized by DAPI staining (blue). Merged red and green channels are shown (merge) and a DIC microscopy image is shown to the right. Scale bar, 5 μm. A dual localisation can be seen for PDEβ consistent with a distinct apical signal predominant in Compound 2–arrested schizonts and a pattern reminiscent of a plasma membrane or inner membrane complex (IMC) localisation in E64-arrested schizonts and in free merozoites
Flueck C, Drought LG, Jones A, Patel A, Perrin AJ, Walker EM, Nofal SD, Snijders AP, Blackman MJ, Baker DA. Phosphodiesterase beta is the master regulator of cAMP signalling during malaria parasite invasion. PLoS Biol. 2019 17(2):e3000154.
Other associated proteins
|PF3D7_0930300||merozoite surface protein 1|
|PF3D7_1222700||glideosome-associated protein 45|
|PF3D7_1321500||3',5'-cyclic nucleotide phosphodiesterase beta|
|PF3D7_1323500||PEXEL protease plasmepsin V|