CLAG3 expression in parasite cultures selected with different concentrations of blasticidin S. Immunofluorescence Assay (IFA) analysis of mature schizont-infected erythrocytes with anti-CLAG3 and anti-AMA1 antibodies in blasticidin S-selected lines and the parental 10G line. DAPI marks parasite nuclei. Anti-AMA1 antibodies were used to identify schizonts sufficiently mature for CLAG3-expression.

Mira-Martínez S, Pickford AK, Rovira-Graells N, Guetens P, Tintó-Font E, Cortés A, Rosanas-Urgell A. Identification of antimalarial compounds that require CLAG3 for their uptake by P. falciparum-infected erythrocytes. Antimicrob Agents Chemother. 2019 pii: AAC.00052-19.