Immunofluorescence Assay (IFA) analysis of mature schizont-infected erythrocytes with anti-CLAG3 and anti-AMA1 antibodies in T3-selected (10G-T3) or unselected 10G cultures. DAPI marks parasite nuclei. Anti-AMA1 antibodies were used to identify schizonts sufficiently mature for CLAG3-expression. The proportion of AMA1-positive infected erythrocytes that are negative for CLAG3 in 10G-T3 or unselected 10G cultures is shown.

Mira-Martínez S, Pickford AK, Rovira-Graells N, Guetens P, Tintó-Font E, Cortés A, Rosanas-Urgell A. Identification of antimalarial compounds that require CLAG3 for their uptake by P. falciparum-infected erythrocytes. Antimicrob Agents Chemother. 2019 pii: AAC.00052-19.