The K119TmATS-GFP and K60Tm(ama1)ATSs chimeric proteins associate with Maurer clefts. Immunofluorescence microscopy of trophozoite stage parasites from 3 different transgenic cell lines with αGFP and αSBP1 as a Maurer cleft resident marker. First image in each row represents the chimera detected with a rabbit αGFP antibody; the second displays the Maurer cleft protein, SBP1; the third DAPI staining of the nuclei; the fourth image is an overlay of all 3; and the last image represents a brightfield image of the infected RBC. First row, 3D7-K119TmATS parasites; second row, D10-K119TmATS parasites; third row, 3D7-K60Tm(ama1)ATSs parasites.
Knuepfer E, Rug M, Klonis N, Tilley L, Cowman AF. Trafficking of the major virulence factor to the surface of transfected P. falciparum-infected erythrocytes. Blood. 2005 105(10):4078-87.
Other associated proteins