Visualizing knob structures at the external and internal surfaces. (A) SEM of an intact CS2-infected RBC (~36 h post-invasion). Zoom shows a representative 1 x 1 μm section. (C) dSTORM imaging of a sheared membrane preparation from CS2-infected RBCs (~30 h post-invasion) labelled with anti-KAHRP (mAb89)
and anti-mouse Alexa-647 secondary antibody. Zoom shows a representative 1 x 1 μm section. Blue arrows: puncta. White arrows: ring structures. (E) Sheared membranes prepared from CS2-infected RBC membranes at 30 h post-invasion imaged by SEM. Zoom shows a representative 1 x 1 μm section. White arrow: Dimpled disk-structure. (F) STORM-SEM showing overlay of physical dimpled disks and anti-KAHRP labelled ring structures (white arrow). Scale bars: (A,C, E, F) 500 nm.
Looker O, Blanch AJ, Liu B, Nunez-Iglesias J, McMillan PJ, Tilley L, Dixon MWA. The knob protein KAHRP assembles into a ring-shaped structure that underpins virulence complex assembly. PLoS Pathog. 2019 May 9;15(5):e1007761.