PF3D7_0731500 erythrocyte binding antigen-175

Right panel: PfPH2 localizes close to the apical tip. IFA showing the distance between the farthest edge of the DAPI and PfPH2 and (Ai) PfEBA175 and (Aii) PfRON4. Two images are shown for EBA175 to demonstrate how much variety can be observed between different cells. Green: PfPH2. Red: EBA175 (Ai) or RON4 (Aii). Blue: DAPI-stained nucleus. Scale bar: 0.5 mm. The bigger the distance between the marker and the DAPI, the more apical a protein likely is. The data show that PfPH2 is farther from the DAPI than PfEBA175 and potentially more than PfRON4/ Left panel: PfPH2 is important for attachment to and subsequent invasion of the red blood cell. A Live-cell microscopy showing that in the presence of 250 nM Rapa, PfPH2-2xFKBP-GFP (GFP) is translocated from the apical pole to the nucleus in the presence of the nuclear mislocalizer. Scale bar represents 5 mm. Blue: DAPI-stained nucleus. BF: bright field.

Ibrahimzadeh Z, Mukherjee A, Crochetière MÈ, Sergerie A, Amiar S, Thompson LA, Gagnon D, Gaumond D, Stahelin RV, Dacks JB, Richard D. A pan-apicomplexan phosphoinositide-binding protein acts in malarial microneme exocytosis. EMBO Rep. 2019 May 16. pii: e47102.

Other associated proteins

PFID Formal Annotation
PF3D7_1116000 rhoptry neck protein 4
PF3D7_1337700 phosphoinositide-binding protein ph2