Generation of PfGRP170-GFP-DD Parasites (HSP110). Asynchronous PfGRP170-GFP-DDD parasites were paraformaldehyde fixed and stained with anti-GFP, anti-PfGRP78 (BiP), and DAPI to visualize the nucleus. Images were taken as a Z-stack using super resolution microscopy and SIM processing was performed on the Z-stacks. Images are displayed as a maximum intensity projection. The scale bar is 2μm. PfGRP170 has a definite ER localization.

Kudyba HM, Cobb DW, Fierro MA, Florentin A, Ljolje D, Singh B, Lucchi NW, Muralidharan V. The ER chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites. Cell Microbiol. 2019 May 14:e13042.