Segmentation is impaired in PfCINCH (P. falciparum coordinator of nascent cell detachment)-deficient parasites. a Airyscan super-resolution micrographs of merozoite plasma membrane and micronemes, stained with antibodies against P. falciparum merozoite surface protein 1 (PfMSP1) and PfAMA1, respectively, in E64-treated schizonts cultivated with and without anhydrotetracycline (ATc). Following the egress trigger, P. falciparum apical membrane antigen 1 (PfAMA1) is translocated from micronemes to the merozoite membrane (bottom panels). b Confocal micrographs of PfGAP45 (IMC marker) and residual PfCINCH over the course of segmentation in PfCINCH-deficient parasites. PfCINCH is visible in schizonts, but forms relatively larger rings that surround multiple nuclei as merozoites are formed. All scale bars 1 μm. Micronemes are present in PfCINCH-deficient parasites and are even discharged upon the egress trigger to the PPM, encircling the poorly segmented daughter cell agglomerates (a). PfGAP45 staining is similar to that observed with PfMSP1 in these cells and demonstrates that the IMC has formed interior to the PPM, which surrounds the daughter cell aggregates (b).
Rudlaff RM, Kraemer S, Streva VA, Dvorin JD. An essential contractile ring protein controls cell division in Plasmodium falciparum. Nat Commun. 2019 10(1):2181
Other associated proteins
|PF3D7_0407800||coordinator of nascent cell detachment CINCH|
|PF3D7_0930300||merozoite surface protein 1|
|PF3D7_1222700||glideosome-associated protein 45|