PfGRP170 (HSP110) Interacts with BiP. (C). In vivo interaction of PfGRP170 and BiP. PfGRP170-GFP-DDD parasites were paraformaldehyde fixed and stained with anti-GFP and anti-BiP. A Proximity Ligation Assay (PLA) was then performed. The scale bar is 5μm. A negative control using anti-GFP and anti-PfPMV is shown in (D). (E). Asynchronous PfGRP170-GFP-DDD parasites overexpressing PfBiP-Ty1were paraformaldehyde fixed and stained with anti-GFP (PfGRP170), anti-Ty1 (PfBiP-Ty1-KDEL), and DAPI to visualize the nucleus. The images were taken with Delta Vision II, deconvolved, and are displayed as a maximum intensity projection. The scale bar is 5μm.
Kudyba HM, Cobb DW, Fierro MA, Florentin A, Ljolje D, Singh B, Lucchi NW, Muralidharan V. The ER chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites. Cell Microbiol. 2019 May 14:e13042.
Other associated proteins