RON3 is essential for the export of proteins into the infected RBC. Ring infected RBCs from RON3loxP v1 (A) and v2 (B) parasites were treated with DMSO or rapamycin (Rapa) for 4 h, washed, and cultured in cRPMI for ~40 to 44 h. The parasites were fixed and stained with antibodies to RON3, KAHRP, RESA, MAHRP1, EXP2, or PTEX150 antibodies. RON3, KAHRP, and EXP2 staining is shown in green, and RESA, MAHRP1, and PTEX150 staining is shown in red. The nuclei staining (4=,6=-diamidino-2-phenylindole [DAPI]) is shown in blue. MAHRP1, KAHRP, or RESA was exported into the DMSO-treated infected RBCs while they appeared to be around the rapamycin-treated parasite. All images are representative of the majority of parasites over several fields and/or of three independent experiments. DIC, differential interference contrast. Bars, 2 mm.
Low LM, Azasi Y, Sherling ES, Garten M, Zimmerberg J, Tsuboi T, Brzostowski J, Mu J, Blackman MJ, Miller LH. Deletion of Plasmodium falciparum Protein RON3 Affects the Functional Translocation of Exported Proteins and Glucose Uptake. MBio. 2019 Jul 9;10(4). pii: e01460-19.
Other associated proteins
|PF3D7_0102200||ring-infected erythrocyte surface antigen|
|PF3D7_0202000||knob-associated histidine-rich protein|
|PF3D7_1252100||rhoptry neck protein 3|
|PF3D7_1370300||membrane associated histidine-rich protein|
|PF3D7_1436300||translocon component PTEX150|