Rab5a shows an aberrant localisation in the second IDC after clindamycin treatment. (A) Representative live-cell images of untreated and clindamycin (5 μM) treated parasites in the second IDC following treatment are
shown. Cell populations were divided into parasites with 6 or fewer nuclei (upper panel) and parasite with more than 6 nuclei (lower panel), as the disruption is clearly most pronounced in trophozoite stage parasites. GFP-Rab5a, green signal; DAPI: parasite nuclei, blue signal; merge of green and blue signal. Scale bar = 5 μm.
Kennedy K, Cobbold SA, Hanssen E, Birnbaum J, Spillman NJ, McHugh E, Brown H, Tilley L, Spielmann T, McConville MJ, Ralph SA. Delayed death in the malaria parasite Plasmodium falciparum is caused by disruption of prenylation-dependent intracellular trafficking. PLoS Biol. 2019 17(7):e3000376.