The CB-EME-labelled F-actin (parasites stably expressing actin-chromobodies tagged with the emerald tag network is disrupted upon genetic excision of pfact1: A. IFA showing the F-actin network in P. falciparum (CB-EME), which stains the same network as an actin antibody (anti-PfAct1), though at slightly different regions , Pearson’s R value = 0.6, compared to R values of 0.2 when the CBEME signal was tested for colocalisation with the DAPI signal. DAPI labels nuclei. F-actin was prominent around the FV of the parasite which is also the basal end of the newly-formed daughter parasites during the final stages of cytokinesis. D. IFA showing apicoplasts (red) in context of the F-actin network (CB-EME, DMSO) and the disruption of the network together with apicoplasts when PfACT1 is deleted (PfAct1cKO). Upon disruption of PfACT1, a defect in apicoplast segregation was apparent.
Felix Stortz J, Del Rosario M, Singer M, Wilkes JM, Meissner M, Das S. Formin-2 drives polymerisation of actin filaments enabling segregation of apicoplasts and cytokinesis in Plasmodium falciparum. Elife. 2019 8. pii: E49030.