Disruption of RAMA leads to selective mislocalisation of some but not all rhoptry proteins. C) The extent of RON protein mis-trafficking depends on the maturity of the RAP-treated RAMAloxP schizonts. Whilst some signal was still detectable in less mature schizonts (those with fewer nuclei), the RON2, RON3 and RON4-specific signal was absent or much reduced in the mature segmented schizonts (examples indicated with arrows). Note that these images are of lower magnification that those shown in the rest of the figure in order to display multiple schizonts in each field. Scale bar, 10 μm. (D) Schizonts of DMSO- and RAP-treated RAMAloxP clone 9C10 were probed with a polyclonal antibody against AMA1 as well as anti-MSP1 antibodies. AMA1 localisation (upper panel) and discharge onto the merozoite surface in mature schizonts (lower panel) was unaffected by RAMA disruption. Scale bar, 2 μm. Parasite nuclei were visualised throughout by staining with DAPI.
Sherling ES, Perrin AJ, Knuepfer E, Russell MRG, Collinson LM, Miller LH,Blackman MJ. The Plasmodium falciparum rhoptry bulb protein RAMA plays an essential role in rhoptry neck morphogenesis and host red blood cell invasion PLoS Pathog. 2019 15(9):e1008049.
Other associated proteins
|PF3D7_0707300||rhoptry-associated membrane antigen|
|PF3D7_1116000||rhoptry neck protein 4|
|PF3D7_1133400||apical membrane antigen 1|
|PF3D7_1252100||rhoptry neck protein 3|
|PF3D7_1452000||rhoptry neck protein 2|