Localization of EXP2 and PVM proteins in ΔEXP1 parasites and interaction analysis. (A) Live cell images of ΔEXP1 (rapalog) and control trophozoites expressing EXP2-GFP. Light blue arrowhead, loop-like protrusions. (C) Live cell images of Bodipy-TR-ceramide labelled ΔEXP1 (rapalog) and control parasites expressing EXP2-GFP. At the beginning of the trophozoite stage, EXP2-GFP displayed a profoundly altered distribution in 75% of the ΔEXP1 cells as evident by the concentration of EXP2 in small regions of the PVM, frequently in loop-like Bodipy-TR-ceramide positive protrusions. In control cells, EXP2-GFP was predominantly found in a circular pattern around the parasite.
Mesén-Ramírez P, Bergmann B, Tran TT, Garten M, Stäcker J, Naranjo-Prado I, Höhn K, Zimmerberg J, Spielmann T. EXP1 is critical for nutrient uptake across the parasitophorous vacuole membrane of malaria parasites. PLoS Biol. 2019 17(9):e3000473
Other associated proteins
|PF3D7_1121600||parasitophorous vacuole membrane antigen QF 116 exported protein 1 circumsporozoite-related antigen|