Upper panel: IFA of compound 2-arrested condΔEXP1 late-stage schizonts show localization of EXP1*-HA at the PVM. α-MSP1 (MSP1) labels the PPM. Nuclei were stained with DAPI; scale bars, 5 μm. IFA of control and ΔEXP1 (rapalog) late-stage gametocytes using α-HA to detect EXP1*-HA and α-Pfg377 (late-stage gametocyte marker). Stage III–V gametocytes lacking EXP1 were detected.

Mesén-Ramírez P, Bergmann B, Tran TT, Garten M, Stäcker J, Naranjo-Prado I, Höhn K, Zimmerberg J, Spielmann T. EXP1 is critical for nutrient uptake across the parasitophorous vacuole membrane of malaria parasites. PLoS Biol. 2019 17(9):e3000473