Conditional regulation of PfPKAc. Widefield imaging of NF54 PfPKAc:loxP schizonts labeled with anti-GAP45, anti-HA, and DAPI. PfPKAc-HA shows partial peripheral and cytoplasmic localization. Scale bars represent 2.5 mm. Immunofluorescence (IF) analysis of control PfPKAc-HA parasites showed a cytoplasmic and slightly peripheral membrane localization of the PfPKAc-HA fusion protein around the developing merozoites compared to PfGAP45. rapamycin led to a specific loss of PfPKAc-HA signal without affecting the localization of PfGAP45.

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