Efficient Shld-1 regulation of N terminally tagged DD (original) tagged proteins in P. falciparum. (A) Plasmid derived from pRM2-GFP for N terminal DD tagging of a reporter gene - here a triple HA tag fused to GFP. This plasmid contains the strong and schizont-specific msp2 promoter. Note that we used a smaller Shld-1 concentration in order to avoid the delay in intraerythrocytic development observed with higher Shld-1 concentrations which would lead to an overall decreased signal due to weaker promoter activity in earlier than schizont stages.
de Azevedo MF, Gilson PR, Gabriel HB, Simões RF, Angrisano F, Baum J, Crabb BS, Wunderlich G. Systematic analysis of FKBP inducible degradation domain tagging strategies for the human malaria parasite Plasmodium falciparum. PLoS One. 2012;7(7):e40981