(A) Co-localization of Nop1 and calpain-GFP (a) or calpain-flag (b). (B) Colocalization of calpain and Nop1 in a purified 3D7 nucleus. A confocal image of sample immunostained with antibodies specific for Pf-Calpain (#35, rabbit) and hNop1 (mouse) is shown. The nucleus was stained with Topro3. (C and D) Enlarged nuclei of parasites expressing small chimeras. Arrows indicate the position of the nucleolus

that in live cells appears clearly negative for the DNA staining.

Russo I, Oksman A, Goldberg DE. Fatty acid acylation regulates trafficking of the unusual Plasmodium falciparum calpain to the nucleolus. Mol Microbiol. 2009 72(1):229-45.