PF3D7_1136900 subtilisin-like protease 2

Expression and OB localization of the PfSUB2-, PfDPAP2- and PfOBC13-GFP fusions in late gametocytes. A, GFP fluorescence

of live gametocytes from the lines indicated. B, IFA experiments with a-GFP (rabbit, 1: 100, green) used alone or in combination with rat a-Pfg377-A2 (1:200 dilution) antibodies on gametocytes from the lines indicated. BF: bright field. Scale bar: 5 mM. The a-GFP antibody produced a granular fluorescence pattern in gametocytes of the pfsub2GFP, pfdpap2GFP and pfobc13GFP lines (left). A different pattern was found instead in the pfpsop12GFP line, with gametocytes showing in some experiments a more condensed cytoplasmic signal, or no signal at all (data not shown). Importantly,

the GFP signals in the gametocytes of 3D7pfsub2GFP,

3D7pfdpap2GFP, and 3D7pfobc13GFP transgenic clones colocalized

with those of the OB marker Pfg377 and of PfGEST (right),

Suárez-Cortés P, Sharma V, Bertuccini L, Costa G, Bannerman NL, Sannella AR, Williamson K, Klemba M, Levashina EA, Lasonder E, Alano P. Comparative Proteomics and Functional Analysis Reveal a Role of Plasmodium falciparum Osmiophilic Bodies in Malaria Parasite Transmission. Mol Cell Proteomics. 2016 15(10):3243-3255.

Other associated proteins

PFID Formal Annotation
PF3D7_1214800 conserved Plasmodium protein, unknown function
PF3D7_1247800 dipeptidyl aminopeptidase 2
PF3D7_1250100 osmiophilic body protein g377
PF3D7_1449000 gamete egress and sporozoite traversal protein, putative