H3K4me3 and H3K9me3 levels following treatment of gametocytes with BIX-01294. (a,b) Detection of H3K4me3 and H3K9me3 marks in gametocytes by indirect immunofluorescence assays. Presence of H3K4me3 and H3K9me3 in gametocytes were detected in the different gametocyte stages (GC stage II-V) via immunolabelling using rabbit anti-H3K4me3 (a) and anti-H4K9me3 (b) (green). Gametocytes were highlighted with mouse antibodies against the gametocyte marker Pfs230 (red). Nuclei were highlighted by Hoechst nuclear stain 33342 (blue). Bar, 5 mm.
Ngwa CJ, Kiesow MJ, Orchard LM, Farrukh A, Llinás M, Pradel G. The G9a Histone Methyltransferase Inhibitor BIX-01294 Modulates Gene Expression during Plasmodium falciparum Gametocyte Development and Transmission. Int J Mol Sci. 2019 20(20). pii: E5087.
Other associated proteins
|PF3D7_0508100||SET domain protein, putative|