TAF1/BDP5 is a nuclear protein essential for transcription during asexual development. (A) Fluorescence and differential interference contrast (DIC) images of parasites in which the endogenous taf1/bdp5 gene is tagged with a sequence encoding 2xFKBP-GFP-2xFKBP tag and carrying a plasmid with the Lyn-FRB-mCherry or Lyn-FRB-T2A-mCherry mislocalizer. Images were taken 24 h after induction of knock sideways of TAF1/BDP5 with rapalog and compared to controls grown without rapalog. Size bars: 5 um; DIC: differential interference contrast; nuclei: DAPI; merge: merged green (GFP) and red (mCherry) channels. Fluorescent life microscopy imaging of the tagged parasites as well as TAF1/BDP5-AID-GFP parasites we used for the IPs revealed weak expression and preferential nuclear localization of the tagged TAF1/BDP5 in particular at ring and trophozoite stages.

Maria Hoeijmakers WA, Miao J, Schmidt S, Toenhake CG, Shrestha S, Venhuizen J, Henderson R, Birnbaum J, Ghidelli-Disse S, Drewes G, Cui L, Stunnenberg HG, Spielmann T, Bártfai R. Epigenetic reader complexes of the human malaria parasite, Plasmodium falciparum. Nucleic Acids Res. 2019 Nov 15. pii: gkz1044.