Ionic imbalance initiated autophagy leads to apoptosis in Plasmodium falciparum, thereby inducing co localization of PfATG8 and PfRAB7. Fluorescent microscopy images of sub-cellular distribution of PfATG8 and PfRAB7 in schizonts stained with anti-PfATG8 rabbit sera and anti-PfRAB7 rat sera. Nuclear DNA was counterstained with DAPI. In scaffold 7 treated parasite, PfATG8 co-localizes with PfRAB7 indicating parasite is undergoing autophagy.

Dangi P, Jain R, Mamidala R, Sharma V, Agarwal S, Bathula C, Thirumalachary M, Sen S, Singh S. Natural Product Inspired Novel Indole based Chiral Scaffold Kills Human Malaria Parasites via Ionic Imbalance Mediated Cell Death. Sci Rep. 2019 9(1):17785.